BBSRC Portfolio Analyser
Award details
Molecular and chemical mechanism underlying human CYP17 catalysis
Reference
C15658
Principal Investigator / Supervisor
Dr P Lee-Robichaud
Co-Investigators /
Co-Supervisors
Institution
University of Sheffield
Department
Chemistry
Funding type
Research
Value (£)
177,252
Status
Completed
Type
Research Grant
Start date
01/02/2002
End date
01/02/2005
Duration
36 months
Abstract
CYP17 is a microsomal protein that is involved in the biosynthesis of glucocorticoids and sex hormones. To achieve this CYP17 catalyses two different generic reactions at the same active site; hydroxylation and carbon-carbon bond cleavage. Both activities require the presence of a redox protein, NADPH-cytochrome P450 reductase, but expression of the second activity is dependent on a third microsomal protein, cytochrome b5. PCR-site-directed mutagenesis, recombinant protein technology, chemical synthesis, 19F-NMR and a variety of biophysical techniques will be used to characterise the binding interaction between b5 and CYP17 and to delineate the molecular mechanism by which b5 subtly modulates the catalytic properties of human CYP17.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search