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Analysis of plant actin-depolymerising factor (ADF) kinase: a calcium dependent or calmodulin- like domain protein kinase

Reference	C15527
Principal Investigator / Supervisor	Professor P Hussey
Co-Investigators / Co-Supervisors	Dr Ellen Allwood
Institution	Durham University
Department	Biological and Biomedical Sciences
Funding type	Research
Value (£)	203,000
Status	Completed
Type	Research Grant
Start date	01/10/2001
End date	31/08/2005
Duration	47 months

Abstract

Analysis to date suggests that calcium-dependent or calmodulin-like domain protein kinase (CDPK) regulates the activity of plant ADF; LIM kinase controls metazoan ADFs. We aim to identify the CDPK(s) that phosphorylate maize and Arabidopsis ADF in cDNA libraries using ADF phosphorylation as the assay. The cDNA clones will be sequenced and compared, and isotype specific antibodies made to the variable domains for immunolocalisation studies. The selected CDPK(s) will be used to establish a link with ADF and the actin cytoskeleton in vivo by analysing Arabidopsis plants expressing CDPK RNA interference transgenes, Arabidopsis mutants with CDPK T- DNA insertions (if identified) and Arabidopsis plants expressing CDPK dominant-positive transgenes. In addition the recombinant CDPK proteins will be characterised in vitro.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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