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Single molecule mechanics and biochemical studies of transcription initiation

Reference	C15315
Principal Investigator / Supervisor	Dr James Hoggett
Co-Investigators / Co-Supervisors	Dr Christoph Baumann, Dr Justin Molloy
Institution	University of York
Department	Biology
Funding type	Research
Value (£)	252,468
Status	Completed
Type	Research Grant
Start date	01/05/2001
End date	01/05/2004
Duration	36 months

Abstract

We shall exploit the potential of single molecule studies to investigate the early stages of transcription initiation and promoter escape by RNA polymerase. We shall compare two polymerases which use a common core enzyme alpha two beta betacience: (1) the sigma 70 holoenzyme which is the paradigm bacterial enzyme; and (2) the sigma 54 holoenzyme which resembles eukaryotic enzymes in requiring enhancer proteins and ATP hydrolysis for activity. We shall use optical tweezer transducers coupled with single molecule fluorescence visualisation to: (1) investigate the relationship between promoter escape and sigma release at different promoters for the sigma 70 enzyme, which does not require enhancers; and (2) for the sigma 54 holoenzyme investigate the consequences of enhancer contact and ATP hydrolysis on transcription initiation, promoter escape and sigma release.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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