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Functional analysis of the mismatch uracil glycosylase (MUG) DNA repair enzyme
Reference
C15052
Principal Investigator / Supervisor
Professor Geoffrey Baldwin
Co-Investigators /
Co-Supervisors
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
195,336
Status
Completed
Type
Research Grant
Start date
17/09/2001
End date
17/09/2004
Duration
36 months
Abstract
The E. coli mismatch uracil glycosylase (MUG) has been shown to cleave uracil, thymine and ethenocytosine from mismatches with guanine. However, it is not clear whether MUG is responsible for repairing these lesions in vivo. The aim of this project is it to investigate the action of MUG with a variety of different substrates both in vitro and in vivo. By using in vitro assays we can determine the binding affinity of MUG for different DNA lesions as well as the rate of N-glycosyl hydrolysis. An in vivo assay will then be used to investigate the action of MUG with the same DNA lesions against different genetic backgrounds. The combination of both in vitro and in vivo assays will allow the cellular function of MUG to be investigated, based upon a detailed understanding of its molecular mechanism.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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