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Imaging natural killer cell immune synapse formation by green fluorescent protein lifetime microscopy

Reference	C14675
Principal Investigator / Supervisor	Professor Daniel Davis
Co-Investigators / Co-Supervisors	Professor Paul Michael William French, Professor David Phillips, Professor Klaus Suhling
Institution	Imperial College London
Department	Biological Sciences
Funding type	Research
Value (£)	323,988
Status	Completed
Type	Research Grant
Start date	01/01/2001
End date	01/01/2004
Duration	36 months

Abstract

Davis recently found that immunosurveillance by human natural killer (NK) cells involves supramolecular organisation of receptors and ligands in discrete membrane microdomains at contacts with target cells or immune synapses. Phillips pioneered time-correlated single photon counting to measure fluorescence lifetimes and French recently developed 2D3D fluorescence lifetime imaging microscopy (FLIM) using a solid-state laser and time-gated image intensifier. We propose to use FLIM of enhanced green fluorescent protein (EGFP)-linked ligands and receptors to characterise the local environment of proteins at NK cell immune synapses. FLIM non-invasively reports on the local environment of EGFP and will determine the role of viscous membrane microdomains, receptor/ligand oligomerisation, and protein/lipid interactions during NK cell immunosurveillance.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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