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Molecular analysis of the chemotaxis system of cyanobacteria and its involvement in cyanobacter-plant symbioses
Reference
C14515
Principal Investigator / Supervisor
Dr David Adams
Co-Investigators /
Co-Supervisors
Institution
University of Leeds
Department
Inst of Molecular & Cellular Biology
Funding type
Research
Value (£)
197,240
Status
Completed
Type
Research Grant
Start date
01/03/2001
End date
01/03/2004
Duration
36 months
Abstract
We have shown that hormogonia, the infective agents in most plant-cyanobacteria symbioses, exhibit positive chemotaxis to exudates of the liverwort Blasia and wheat seedlings. The objectives of this project are to: 1. clone methyl-accepting chemotaxis protein (MCP)-encoding genes from the symbiotically competent Nostoc 29133; 2. inactivate mcp genes by insertional mutagenesis and characterise the mutants in terms of motility, chemotaxis and ability to form N2-fixing associations with plants; 3. localise MCPs in Nostoc filaments using immunogold electron microscopy; 4. use transposon-lux mutagenesis of Nostoc 29133 and low-light imaging of the mutants to identify genes that are responsive to Blasia and wheat exudates and to other attractants.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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