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Functional analysis of protein sorting signals of barley phytepsin
Reference
C14502
Principal Investigator / Supervisor
Dr Jurgen Denecke
Co-Investigators /
Co-Supervisors
Institution
University of Leeds
Department
Plant Biochemistry & Biotechnology
Funding type
Research
Value (£)
172,568
Status
Completed
Type
Research Grant
Start date
01/01/2002
End date
01/01/2005
Duration
36 months
Abstract
We have established that barley phytepsin contains two vacuolar sorting signals, one for transport to the lytic vacuole (VSS1) and a second for a storage vacuole (VSS2). VSS1 is situated in the plant specific insert (PSI), a 104 amino acid insert with homology to mammalian saposin C which appears to be completely unrelated to any known vacuolar sorting signals. VSS2 is situated near the C-terminus of the protein. We propose to identify the sorting determinants for VSS1 within the PS1 sequence as well as determine how PSI directs barley phytepsin into COPII transport vesicles for ER export. In addition, we want to identify the machinery responsible for the interaction with VSS2 because phytepsin Delta PSL, which lacks VSS1, is exported from the ER in a COPII independent fashion.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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