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Molecular characterisation of SIF1: a novel regulator of eukaryotic protein synthesis initiation factor eIF2B
Reference
C14368
Principal Investigator / Supervisor
Professor Graham Pavitt
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
183,984
Status
Completed
Type
Research Grant
Start date
11/06/2001
End date
11/06/2004
Duration
36 months
Abstract
Eukaryotic protein synthesis initiation factor eIF2B is the essential guanine-nucleotide exchange factor for eIF2. eIF2B is known to regulate protein synthesis in response to cellular stresses. We will characterise a novel regulator of eIF2B (termed SIF1) that specifically suppresses the eIF2B catalytic defect of yeast eIF2B mutations but does not alter the previously characterised response of eIF2B to amino acid starvation. The priorities are: 1) To clone the SIF1 gene by complementation using a yeast genetic screen. 2) Biochemical analysis of the effects of the sif1 mutation on eIF2B activity in vitro. 3) Genetic and biochemical experiments using modern molecular tools to determine the function of SIF1 in translation initiation.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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