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The roles of periplakin and creatine kinase B in the distribution and function of the Mu opioid receptor

Reference	C14356
Principal Investigator / Supervisor	Professor Graeme Milligan
Co-Investigators / Co-Supervisors
Institution	University of Glasgow
Department	IBLS Division of Biochemistry & Molecula
Funding type	Research
Value (£)	210,720
Status	Completed
Type	Research Grant
Start date	01/02/2001
End date	31/01/2004
Duration	36 months

Abstract

There is currently great interest in the capacity of proteins which interact with G protein-coupled receptors (GPCRs) to alter their signalling properties and regulation. Using a yeast two-hybrid screen with a human brain cDNA library we have identified interactions between the C-terminal tail of the Mu opioid receptor (MOR) and both periplakin and creatine kinase B. We will provide a detailed analysis of the sites of these interactions, analyse the pattern of expression and co-localisation of these proteins with the MOR in brain and analyse differences in regulation, pharmacology and function of the MOR in cell lines in which it is expressed either in concert with or in the absence of these two binding partners. Such studies will provide the first analysis of the effects of proteins, not viewed classically as GPCR interacting partners, to modulate the function of a key GPCR involved in analgesia and drug dependence.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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