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A proteomics based approach applied to the definition of IgE-receptor mediated signalling pathways in mast cells

Reference	C14257
Principal Investigator / Supervisor	Mr P Strobl
Co-Investigators / Co-Supervisors	Dr Kathleen Carroll
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	259,864
Status	Completed
Type	Research Grant
Start date	01/01/2001
End date	30/06/2004
Duration	42 months

Abstract

2-D protein electrophoresis and mass spectrometry will be applied to gain an integrated description of IgE- receptor mediated signalling pathways using the RBL cell line as a model of primary mast cell function. Chemical/biochemical techniques will be employed to identify post-translational modifications, such as phosphorylation and lipidation, and problems associated with the recovery of hydrophobic proteins. The standardisation of these methods forms the basis to study signalling events in human mast cells, where phenotype definition in terms of functional protein expression can be expected to identify novel targets for therapeutic interventions in allergic and inflammatory diseases. Our study is very timely and complements ongoing efforts to define signalling events in B-cells and myocytes by 2-D protein arrays.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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