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A molecular genetics approach to identify trans-acting factors in the assembly of cytochrome P450s
Reference
C14240
Principal Investigator / Supervisor
Professor Alison Smith
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Plant Sciences
Funding type
Research
Value (£)
218,868
Status
Completed
Type
Research Grant
Start date
01/09/2001
End date
01/09/2004
Duration
36 months
Abstract
The aim is to identify genes encoding proteins involved in the assembly of functional cytochrome P450s in yeast and in Arabidopsis. Despite the importance of these proteins, nothing is known about how the haem cofactor, made in the mitochondrion (and/or plastid in plants) is delivered to the apoprotein in the ER. Two approaches will be followed: (1) identification of yeast mutants defective in the process, followed by isolation of the yeast and Arabidopsis genes by complementation; (2) use of the yeast two-hybrid system to isolate cDNAs for Arabidopsis proteins which interact with the terminal haem biosynthesis enzyme on the one hand, and cytochrome P450 apoproteins on the other.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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