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The signal for perinuclear mRNA localisation and association with the cytoskeleton
Reference
C14236
Principal Investigator / Supervisor
Professor John Hesketh
Co-Investigators /
Co-Supervisors
Institution
Newcastle University
Department
Agriculture Food and Rural Development
Funding type
Research
Value (£)
184,592
Status
Completed
Type
Research Grant
Start date
13/08/2001
End date
13/08/2004
Duration
36 months
Abstract
mRNA localisation is a novel targeting mechanism of importance in determining where proteins are synthesised in cells. Perinuclear mRNA localisation has recently been linked to subsequent import of proteins into the nucleus. The aims of this project are to define the signals within 3' untranslated regions (3'UTR) that are responsible for perinuclear localisation, and then to formulate a consensus structure and identify novel genes with similar signals. The secondary structure in the 3'UTR of c-myc and metallothionein mRNAs will be defined by combining chemical probing and enzymatic cleavage techniques for RNA structure determination with mutagenesis and determination of RNA targeting using reporter gene constructs transfected into fibroblasts. Computer techniques will then be used to search databases for similar structures and so identify novel localised mRNAs.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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