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Molecular mechanisms of gating in calcium channels: a comparative study of low and high voltage-operated channels
Reference
C14234
Principal Investigator / Supervisor
Professor Dennis Wray
Co-Investigators /
Co-Supervisors
Institution
University of Leeds
Department
Institute of Membrane & Systems Biology
Funding type
Research
Value (£)
197,324
Status
Completed
Type
Research Grant
Start date
01/04/2001
End date
01/10/2004
Duration
42 months
Abstract
Molecular mechanisms of gating in calcium channels will be studied: in particular we will investigate the molecular basis of differences in activation voltage between recently-cloned low-voltage activated T type channels and high-voltage activated L type channels. Interdisciplinary techniques of molecular biology and electrophysiology will be used in an international collaborative study. Calcium channel mutants will be expressed in oocytes, and two-electrode voltage-clamp recordings will be made. We will make chimaeric mutants, firstly swapping domains I to IV between these subunits, then swapping the S4 regions. We will also carry out cysteine-scanning mutagenesis to determine movement of the S4 region during activation by depolarisation.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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