Award details

A novel family of flavocytochrome acrylate reductases from Shewanella MR-1

Reference	C13914
Principal Investigator / Supervisor	Professor Graeme Reid
Co-Investigators / Co-Supervisors	Professor Stephen Chapman
Institution	University of Edinburgh
Department	Inst of Cell and Molecular Biology
Funding type	Research
Value (£)	176,968
Status	Completed
Type	Research Grant
Start date	01/10/2000
End date	01/10/2003
Duration	36 months

Abstract

We have identified a novel family of enzymes by analysis of the genome sequence of Shewanella. These are periplasmic flavocytochromes that are closely related to the fumarate reductase from the same organism but which appear, from structural analysis of the active sites, to be designed to react with mono- rather than di-carboxylate substrates. We shall identify the natural substrates for each of the three enzymes by identifying inducers of expression, by determining the consequences of gene knock-out and by biochemical analysis of the proteins isolated from Shewanella. Detailed enzymological studies will enable us to determine how substrate specificity is controlled is these and related enzymes.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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