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RNA-protein interactions involved in perinuclear localisation of c-myc mRNA

Reference	C13737
Principal Investigator / Supervisor	Professor John Hesketh
Co-Investigators / Co-Supervisors
Institution	Newcastle University
Department	Agriculture Food and Rural Development
Funding type	Research
Value (£)	192,148
Status	Completed
Type	Research Grant
Start date	01/10/2001
End date	01/10/2004
Duration	36 months

Abstract

mRNA trafficking is a novel targeting mechanism of potential importance in subcellular organisation. Our recent evidence indicates that perinuclear mRNA localisation influences some nuclear-cytoplasmic protein trafficking. The aim of this project is to define the protein-RNA interactions that lead to perinuclear mRNA localisation. A protein binding to the 86nt localisation signal in the 3' untranslated region of c-myc mRNA will be isolated using RNA affinity methods. Having identified the protein, the gene will be cloned using a PCR approach. Recombinant protein will be made in bacteria and RNA-binding domains defined by UV cross-linking and sequencing. Antibodies will be raised and immunological methods, combined with RTPCR and gel shift assays, used to study the subcellular distribution of the protein and its interactions with RNAs and the cytoskeleton.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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