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The biogenesis of integral cytoplasmic membrane proteins
Reference
C13720
Principal Investigator / Supervisor
Professor Michael Ehrmann
Co-Investigators /
Co-Supervisors
Institution
Cardiff University
Department
School of Biosciences
Funding type
Research
Value (£)
157,640
Status
Completed
Type
Research Grant
Start date
19/02/2001
End date
19/02/2004
Duration
36 months
Abstract
The involvement of the general targeting factor SecA in the biogenesis of complex cytoplasmic membrane proteins is a matter of debate. To address this question, we developed a new method, Target Directed Proteolysis (TDP) to inactivate essential proteins in vivo. TDP uses Tobacco Etch Virus NIa (TEV) protease, which recognises a seven amino acid consensus sequence. Because of its distinct specificity, TEV protease can be expressed in the cytoplasm without interfering with viability. SecA, which is not a natural substrate of TEV protease, is proteolysed if it carries the appropriate cleavage site. We will determine the consequences of proteolytic inactivation of SecA on membrane insertion in vivo.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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