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Structural analysis of K+-dependent gating in the yeast model YKCl K+ channel

Reference	C13599
Principal Investigator / Supervisor	Professor Michael Blatt
Co-Investigators / Co-Supervisors
Institution	University of Glasgow
Department	Environmental and Evolutionary Biology
Funding type	Research
Value (£)	205,844
Status	Completed
Type	Research Grant
Start date	15/07/2001
End date	21/06/2005
Duration	48 months

Abstract

Gating of the yeast YKCl K+ channel, like many outward-rectifying K+ channels from plants, shows an unusual sensitivity to extracellular K+ as well as voltage. The effect is analogous to animal anomalous rectifiers, but the mechanism clearly differs. YKCl includes two pore loops and lacks the archetypal voltage sensor of Kv K+ channels. Our previous work identified two residues at either end of each pore loop that separately affect two K+-dependent gating events. Structural alignments suggest these sites may contribute to two K+-binding sites on the outer surface of the channel. Funds are requested to systematically map these, and additional sites to develop an understanding of the molecular mechanism of these K+ gates.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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