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Insect virus internal ribosome entry sites; characterisation and assessment of utility in protein expression systems

Reference	C13593
Principal Investigator / Supervisor	Professor Lisa Roberts
Co-Investigators / Co-Supervisors	Professor Graham Belsham
Institution	University of Surrey
Department	Health and Medical Sciences
Funding type	Research
Value (£)	185,800
Status	Completed
Type	Research Grant
Start date	08/01/2001
End date	08/01/2004
Duration	36 months

Abstract

Rhopalosiphum padi virus (RhPV) is an insect picorna-like virus. We have recently demonstrated that the 5' UTR of RhPV contains an Internal Ribosome Entry Site (IRES) element. IRES elements have been found in all mammalian picornavirus genomes. The RhPV IRES functions efficiently in the rabbit reticulocyte lysate in vitro translation system and we have also demonstrated efficient activity in the wheat germ translation system. No other IRES have been shown to work in this system to date. This study aims to characterise the 5' IRES of RhPV in comparison with other insect virus UTRs using a range of in vitro and cellular systems. The potential application of this IRES to insect cell protein expression systems will be explored.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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