Award details

The contribution of heparanase to angiogenesis

Reference	C13564
Principal Investigator / Supervisor	Professor Gordon Jayson
Co-Investigators / Co-Supervisors	Professor Paul Brenchley, Dr Ian Noel Hampson
Institution	The University of Manchester
Department	Medical and Human Sciences
Funding type	Research
Value (£)	168,604
Status	Completed
Type	Research Grant
Start date	01/02/2001
End date	01/02/2004
Duration	36 months

Abstract

We will assess the contribution to angiogenesis of heparanase, a b- endoglycosidase implicated in invasion and metastasis, by transfecting heparanase cDNA into endothelial and carcinomatous cell lines that will be characterised in a novel ELISA assay for heparanase. Through the use of characterised oligosaccharides that inhibit bFGF and/or heparanase the contribution of heparanase to angiogenesis will be assessed in 3-dimensional in vitro systems and hollow fibre models, to distinguish the angiogenic from the invasive effects of heparanase. A novel mechanistic assay will be used to determine whether heparanase contributes to angiogenesis through depolymerisation of the extracellular matrix or through release of bFGF.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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