Award details

Amphipathic helix formation: a new paradigm for proteinase inhibition

Reference	C13544
Principal Investigator / Supervisor	Professor John Kay
Co-Investigators / Co-Supervisors	Dr Lowri Phylip
Institution	Cardiff University
Department	School of Biosciences
Funding type	Research
Value (£)	190,747
Status	Completed
Type	Research Grant
Start date	01/10/2000
End date	31/03/2005
Duration	54 months

Abstract

A previous ROPA award enabled us to show that a small protein from yeast inhibited its target aspartic proteinase by a totally unprecedented mode of action. The unstructured free inhibitor forms an amphipathic alpha-helix upon contact with the active site of the target enzyme. The proposed project will examine the interactions between these two macromolecules and elucidate how the proteinase plays the role of a folding template, stabilising the helical conformation in the inhibitor but resulting in turn in the potent, specific blockage of the proteolytic activity. This will enable re-targeting of the inhibitory activity against essential aspartic proteinases from plant and human pathogens.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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