Award details

The bioenergetics and biosynthesis of the bacterial nitric oxide reductase

Reference	C13451
Principal Investigator / Supervisor	Professor David Richardson
Co-Investigators / Co-Supervisors	Dr S Spiro, Dr Nicholas James Watmough
Institution	University of East Anglia
Department	Biological Sciences
Funding type	Research
Value (£)	197,816
Status	Completed
Type	Research Grant
Start date	01/10/2000
End date	30/04/2004
Duration	43 months

Abstract

The bacterial respiratory nitric oxide reductase (NOR) is an ancient member of the family of respiratory heme-copper oxidases that includes mitochondrial cytochrome aa3, and plays an important role in environmental denitrification and detoxification of NO. Unlike the heme-copper oxidases, NOR does not pump protons. It also has a non-heme Fe rather than copper in the heme- metal dinuclear active site. We have successfully expressed the P. denitrificans nitric oxide reductase in Escherichia coli and will use this system to study: 1) the mechanism of proton uptake in NOR; 2) the role of conserved glutamate residues in modulating the properties of the catalytic site; and 3) the role of accessory NOR proteins in NOR assembly and stability.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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