BBSRC Portfolio Analyser
Award details
Exploitation of the conversion of a thermostable c-type cytochrome to a b-type for the study of c-type cytochrome folding and its biogenesis process in E. coli
Reference
C13443
Principal Investigator / Supervisor
Professor Stuart John Ferguson
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Biochemistry
Funding type
Research
Value (£)
173,744
Status
Completed
Type
Research Grant
Start date
01/05/2001
End date
30/04/2004
Duration
36 months
Abstract
We will exploit our recent success in converting, using an unusual cytoplasmic assembly system, a small (MWt, 8 kDa) thermostable cytochrome c into a b-type cytochrome, and the reconstitution of the latter from the apo protein plus haem, to study: 1) the structural consequences of losing the two thioether bonds between polypeptide and haem that characterise a c-type cytochrome; 2) the consequences of losing only one of these bonds; 3) the properties of the apo protein which allow it to recognise haem and rapidly refold to the holo form; 4) the reason why the single cysteine variants of the cytochrome are not recognised by the Ccm cytochrome c biogenesis system of E. coli; and (5) why the overexpression of the ccm genes results in inhibition of the production of the AXXAH, CXXAH and AXXCH variants but enhances the formation of the CXXCH protein.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search