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Catalysis of the light-driven step in chlorophyll biosynthesis by protochlorophyllide reductase
Reference
C13435
Principal Investigator / Supervisor
Krebs Professor of B Christopher Hunter
Co-Investigators /
Co-Supervisors
Institution
University of Sheffield
Department
Molecular Biology and Biotechnology
Funding type
Research
Value (£)
156,292
Status
Completed
Type
Research Grant
Start date
10/04/2000
End date
10/04/2003
Duration
36 months
Abstract
The aim of this work is to combine mutagenesis, stopped-flow methodology and spectroscopy to provide a full kinetic characterisation of protochlorophyllide reductase (POR), which catalyses the light-dependent step in chlorophyll biosynthesis. This is the first time that large quantities of this fascinating enzyme have become available for detailed enzymological studies. The specific objectives are to examine: 1) binding of NADPH and its role in controlling the aggregation state of POR; 2) the binding of Pchlide to this enzyme, and to search for enzyme-bound intermediates of Pchlide reduction, and 3) to initiate structural studies on this enzyme using a combination of site- directed mutagenesis, X-ray crystallography, and NMR.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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