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Genetic and functional characterisation of the cGMP-dependent protein kinase family
Reference
C13431
Principal Investigator / Supervisor
Professor Shireen Davies
Co-Investigators /
Co-Supervisors
Professor Julian Dow
Institution
University of Glasgow
Department
Molecular Genetics
Funding type
Research
Value (£)
203,436
Status
Completed
Type
Research Grant
Start date
01/12/2000
End date
01/12/2003
Duration
36 months
Abstract
cGMP-dependent protein kinases (cGKs) are major effectors of nitric oxide signalling but little is known of their role in epithelial function. cGK function will be defined in an organotypic context using targeted ectopic gene expression in a genetic model epithelium, the Drosophila Malpighian tubule. Physiological function of cGK will be assessed by secretion assays of cGK mutant tubules; tubule-specific transcripts of cGK will be determined and functionality assessed by phenotypic reversion of mutant tubules upon ectopic expression of cGK transcripts using the GAL4-UASG system; cell-specific interactions between cGK and calcium signalling will be defined in mutant and revertant backgrounds using targeted aequorin expression. This work will allow functional assessment of cGK genes in genetically defined cell subtypes, presently possible only in Drosophila.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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