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Use of an in vivo fluorescence assay and dominant genetics to investigate membrane traffic in Arabidopsis

Reference	C13425
Principal Investigator / Supervisor	Professor Ian Moore
Co-Investigators / Co-Supervisors	Dr Henri Batoko, Professor Mark Fricker, Professor Christopher Hawes
Institution	University of Oxford
Department	Plant Sciences
Funding type	Research
Value (£)	200,832
Status	Completed
Type	Research Grant
Start date	01/05/2000
End date	04/03/2004
Duration	46 months

Abstract

We describe a rapid assay, based on GFP accumulation, that allows us to characterise membrane trafficking defects induced by dominant transgenes in transfected tobacco cells. We will establish this assay in Arabidopsis and use ratiometric measurements of spectral variants of GFP to make quantitative measurements. Using this assay we will investigate the involvement in ER-Golgi traffic of two subclasses of the Arabidopsis Rab GTPase family with isoforms that appear to be specific to higher plants. A complementation assay will be used to test the hypothesis that these isoforms have distinct cellular functions.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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