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Characterisation of archaeal Holliday junction resolving enzymes and their role in homologous recombination

Reference	C13364
Principal Investigator / Supervisor	Prof. Malcolm White
Co-Investigators / Co-Supervisors	Professor Mamuka Kvaratskhelia
Institution	University of St Andrews
Department	Biology
Funding type	Research
Value (£)	198,295
Status	Completed
Type	Research Grant
Start date	01/10/2000
End date	01/10/2003
Duration	36 months

Abstract

Holliday junctions arising during homologous recombination are resolved by structure-specific endonucleases. We have identified new junction resolving enzymes with novel mechanisms and properties in the thermophilic crenarchaeote Sulfolobus solfataricus. We will characterise these enzymes using extensive enzymological, biophysical and structure-function studies. The archaeal recombination pathway will be reconstituted using purified proteins, and we will identify interacting proteins by affinity and 2-hybrid techniques. This work will represent a major advance in our understanding of the mechanism of Holliday junction resolution, and of homologous recombination in the archaea, with implications for the equivalent process in eukaryotes.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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