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Role of protein S-thiolation as an antioxidant defence in yeast
Reference
C13319
Principal Investigator / Supervisor
Professor Christopher Grant
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
175,348
Status
Completed
Type
Research Grant
Start date
23/10/2000
End date
23/10/2003
Duration
36 months
Abstract
This project aims to understand protein S-thiolation, in which proteins undergo reversible oxidation to form mixed disulphides with low molecular weight thiols. Cells are exposed to reactive oxygen species during normal aerobic metabolism and following exposure to radical- generating compounds, but proteins can be protected against irreversible oxidation by protein S-thiolation. The yeast Saccharomyces cerevisiae will be used as a model eukaryote to determine the range of proteins modified during different growth phases and following exposure to various stress conditions. The glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase has been identified as the major target of thiolation and will be used to analyse the regulation of the process. In particular, the roles of the glutaredoxin and thioredoxin systems, which have been implicated in sulphydryl regulation, will be determined.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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