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Identification of the specific genes responsible for pollen tube directional growth
Reference
C13167
Principal Investigator / Supervisor
Professor Tony Trewavas
Co-Investigators /
Co-Supervisors
Institution
University of Edinburgh
Department
Inst of Cell and Molecular Biology
Funding type
Research
Value (£)
158,872
Status
Completed
Type
Research Grant
Start date
01/09/2000
End date
01/09/2003
Duration
36 months
Abstract
Spatially localised manipulations of a tip based calcium gradient controls subsequent pollen tube orientation and these effects are in part mediated by calmodulin. Pollen tubes will be transformed using the biolistic gun with specific cDNA constructs which express mutant plant calmodulins. Alterations in hydrophobic binding regions and in specific regions of the N and C terminus putatively involved in calmodulin regulation of channels, kinase and calcium ATPase should enable construction of a calmodulin mutation fingerprint which will be used to identify critical calmodulin binding proteins by differential overlay.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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