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Micro-beam two photon infrared hydroxyl radical generation for the study of intracellular oxidative stress

Reference	C13149
Principal Investigator / Supervisor	Professor Roger Hugh Bisby
Co-Investigators / Co-Supervisors	Professor Stanley Botchway, Professor Anthony Parker
Institution	University of Salford
Department	Res Inst for the Built and Human Env
Funding type	Research
Value (£)	100,384
Status	Completed
Type	Research Grant
Start date	01/12/2000
End date	01/12/2002
Duration	24 months

Abstract

Localised intracellular oxidative stress will be initiated and studied using confocal laser microscopy. Hydroxyl radicals will be generated by two-photon photocleavage of N- hydroxypyridine-thiones, employing a near infra-red microbeam of subcellular dimensions. The volume of intracellular hydroxyl radical generation will be determined using coumarin carboxylate as a probe yielding fluorescent 7-hydroxycoumarin carboxylate. Cellular damage will be assessed by cell viability, damage to DNA and membranes and changes in calcium levels. The latter two will be mapped directly with the confocal microscope. The project will also involve synthesis and characterisation of new N-hydroxypyridinethiones (Salford) and instrumental development (RAL).

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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