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Genetic analyses of chloroplast protein import in Arabidopsis thaliana
Reference
C12976
Principal Investigator / Supervisor
Professor Paul Jarvis
Co-Investigators /
Co-Supervisors
Institution
University of Leicester
Department
Biology
Funding type
Research
Value (£)
200,648
Status
Completed
Type
Research Grant
Start date
12/06/2000
End date
12/06/2003
Duration
36 months
Abstract
The research will enhance understanding of mechanisms for translocating nucleus- encoded proteins into chloroplasts, building on the findings of earlier work (Science, 1998, 282: 100- 103). We will identify new translocon components and elucidate the functionality of previously identified proteins. Two complementary strategies will be employed: (1) Import- defective mutants will be identified by their inability to translocate a chloroplast-specific, negatively-selectable marker. We are collaborating with researchers at the John Innes Centre on this, and have so far identified 3 putative import mutants. Cloning of two novel loci will be attempted. (2) Suppressor mutants able to translocate a chloroplast-specific, positively- selectable marker with an import-defective transit peptide will be isolated. Substantial preliminary experimentation will be necessary in this case.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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