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The role of phosphoinositide 3-kinase(s) in chemokine mediated signal transduction and chemotaxis

ReferenceC12884
Principal Investigator / Supervisor Professor Stephen Ward
Co-Investigators /
Co-Supervisors
Institution University of Bath
DepartmentPharmacy and Pharmacology
Funding typeResearch
Value (£) 152,304
StatusCompleted
TypeResearch Grant
Start date 05/06/2000
End date 05/06/2003
Duration36 months

Abstract

Several chemokines are now known to activate the PI3K signalling pathway which has been linked to the triggering of a diverse array of cellular responses. Our preliminary studies have indicated that the CXC chemokine Stromal cell-Derived Factor (SDF-1) stimulates the accumulation of D-3 phosphoinositides (the products of PI3K activation), whilst PI3K inhibitors prevent SDF-1 stimulated chemotaxis. This study proposes to identify (i) the class of PI3K responsible for the formation of these D-3 lipids and (ii) the downstream biochemical and functional targets of this pathway, using dominant negative mutants of the p85/p110 heterodimeric PI3K and the Gi-coupled p110gamma in a controlled inducible gene expression system.

Summary

unavailable
Committee Closed Committee - Biochemistry & Cell Biology (BCB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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