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A molecular analysis of ribosomal frameshifting
Reference
C12882
Principal Investigator / Supervisor
Professor Ian Brierley
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Pathology
Funding type
Research
Value (£)
271,442
Status
Completed
Type
Research Grant
Start date
01/01/2000
End date
01/01/2003
Duration
36 months
Abstract
This project will investigate the process of ribosomal frameshifting on messenger RNAs. The point in elongation when frameshifting occurs and the involvement of modified tRNAs in the process will be studied by in vitro translation of frameshift reporter transcripts in mRNA- and tRNA-dependent in vitro translation systems. The RNA pseudoknot component will be characterised by RNA structure mapping and crystallography. Further, a yeast three hybrid screen will be used to identify cellular proteins that bind to pseudoknots. These studies will contribute both to our understanding of the mechanism of frameshifting and to our knowledge of RNA pseudoknot structure and function.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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