Award details

Bioelectrochemistry of flavocytochrome c3

Reference	C12775
Principal Investigator / Supervisor	Professor Stephen Chapman
Co-Investigators / Co-Supervisors	Dr Stephen Attwood, Professor Graeme Reid
Institution	University of Edinburgh
Department	Sch of Chemistry
Funding type	Research
Value (£)	187,831
Status	Completed
Type	Research Grant
Start date	01/03/2000
End date	01/06/2003
Duration	39 months

Abstract

The 3-dimensional structure of the multi-centre redox enzyme Flavocytochrome c3 is now known to a resolution of 1.8 angstrom. We shall use protein film voltammetry (PFV) to probe both intramolecular electron transfer and substrate reduction in this enzyme. PFV provides a detailed picture of the redox chemistry in both the time and potential domains; integrating thermodynamics and kinetics in a single set of experiments. A unique aspect of the work that we will be able to probe the chemistry that occurs at the FAD during turnover. Any interaction that perturbs the reduction potential of the FAD or gates electron transfer through the active site will be revealed, and the redox-state dependence and timescale of the processes will be measured.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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