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Award details
Biochemical studies of native and recombinant N-methyl-D-aspartate receptors
Reference
C12756
Principal Investigator / Supervisor
Professor Frances Stephenson
Co-Investigators /
Co-Supervisors
Institution
University College London
Department
Dept of Pharmaceutical and Biological Ch
Funding type
Research
Value (£)
51,570
Status
Completed
Type
Research Grant
Start date
01/12/1999
End date
01/12/2000
Duration
12 months
Abstract
This proposal aims to complete work on the determination of the quaternary structure and subunit composition of the N-methyl-D-aspartate subclass of excitatory neurotransmitter receptor. Thus, the NR1-4b subunit will be epitope-tagged with both FLAG and c- myc tags with the retention of wild-type NR1-4b properties. The NR1 subunits per NMDA receptor will be determined by immunoaffinity purification and characterisation of wild- type/epitope- tagged NMDA receptors expressed transiently in mammalian cells. Verification of the results will be carried out by biotinylation of cell surface cloned NMDA receptors followed by their affinity purification and detection by streptavidin/horseradish peroxidase and NMDA receptor subunit-specific antibodies. Characterisation of native receptors using the NR2B- selective ligand, [3H]Ro 25-6981, will be completed.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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