Award details

Characterisation of a novel protein C3-beta a putative GABAa receptor binding protein

Reference	C12628
Principal Investigator / Supervisor	Professor Frances Stephenson
Co-Investigators / Co-Supervisors
Institution	University College London
Department	Dept of Pharmaceutical and Biological Ch
Funding type	Research
Value (£)	85,272
Status	Completed
Type	Research Grant
Start date	01/04/2000
End date	01/10/2001
Duration	18 months

Abstract

The proposal is to study the interaction between GABAa receptors and the novel protein C3 beta, identified by the yeast two-hybrid system as interacting with the GABAa receptor beta2 subunit intracellular loop. The full length sequence of C3 beta will be obtained. Anti- C3 beta antibodies will be raised and used with anti- GABAa receptor antibodies to study the GABAa receptor and C3 beta association in various in vitro experimental paradigms. The tissue, cellular and sub-cellular distributions of C3 beta and the GABAa receptor beta 2 subunit that mediate the coassociation will be determined by deletion/point mutation. The C3 beta clone, a modified beta 2 subunit intracellular loop and other GABAa receptor intracellular loops will be used as 'baits' to identify downstream interacting proteins that may be important for regulation of either C3 beta or receptor activity.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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