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Analysis of the role of sec6/8 complex in exocytosis in insulin-sensitive cells

Reference	C12621
Principal Investigator / Supervisor	Professor Gwyn William Gould
Co-Investigators / Co-Supervisors
Institution	University of Glasgow
Department	IBLS Division of Biochemistry & Molecula
Funding type	Research
Value (£)	173,350
Status	Completed
Type	Research Grant
Start date	01/04/2000
End date	01/10/2003
Duration	42 months

Abstract

Many of the proteins involved in exocytosis are conserved from yeast to mammals, 7 of the 12 late acting SEC mutants in yeast have only poorly defined mammalian counterparts, but recent studies have identified a complex of 6 of these proteins which control vesicle docking in exocytosis (the 'Exocyst'). Mammalian homologues of two such proteins, rsec6 and rsec8, are widely expressed and we have shown that insulin regulates the subcellular distribution of rsec8 in fat cells; the role of this complex in exocytosis in mammalian cells is not defined. We propose that the rsec6/8 complex is important in insulin regulated exocytosis, and that this complex may be a site for insulin-specific regulation of trafficking. We will examine the role of this complex in a range of membrane trafficking events in insulin- sensitive 3T3-L1 adipocytes by expressing wild-type and mutant sec6 and sec8 proteins in these cells.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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