BBSRC Portfolio Analyser
Award details
Structural and functional analysis of the specialised BRCT domain of E. coli DNA ligase
Reference
C11893
Principal Investigator / Supervisor
Dr Richard Bowater
Co-Investigators /
Co-Supervisors
Institution
University of East Anglia
Department
Biological Sciences
Funding type
Research
Value (£)
108,356
Status
Completed
Type
Research Grant
Start date
01/02/2000
End date
01/02/2002
Duration
24 months
Abstract
BRCT domains are present in a range of DNA repair proteins and their mutation is linked to important human diseases, including breast cancer. Frequently, these motifs specify essential protein- protein contacts, which occur via unknown mechanisms. This project will characterise an unusual BRCT domain from E. coli DNA ligase. Mutagenesis studies will identify the role of specific residues within this motif. Full-length and fragments of DNA ligase will be purified and their ability to bind to DNA and other proteins will be analysed biochemically. Yeast two-hybrid and co- immunoprecipitation assays will characterise interactions occurring in vivo. The ease of genetic manipulations within E. coli is a major advantage for analysis of BRCT domains. In addition, these studies advance our knowledge of prokaryotic DNA ligases, a target for novel antibiotics.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search