Award details

Elucidating the structure of photosystem two by cryoelectron microscopy and single particle analyses

Reference	C11886
Principal Investigator / Supervisor	Professor James Barber
Co-Investigators / Co-Supervisors	Dr Jon Nield, Professor Marin Van Heel
Institution	Imperial College London
Department	Biological Sciences
Funding type	Research
Value (£)	204,841
Status	Completed
Type	Research Grant
Start date	16/10/1999
End date	16/01/2003
Duration	39 months

Abstract

The purpose of this work programme is to obtain 3D structures of photosystem II (PSII) at 10 or better using cryoelectron microscopy and single particle analyses. We will (1) Improve the resolution of the spinach LHCII-PSII supercomplex structure to 10 . (2) Determine the 3D structure of the LHCII-PSII supercomplex from Chlamydomonas reinhardtii to the highest resolution possible and compare it with the structure of a similar supercomplex depleted of the Psbl protein, to identify the location of this subunit. (3) Determine the 3D structure of an allophycocyanin-PSII supercomplex isolated from Synechococcus elongatus. (4) Compare all three structures to highlight similarities and differences. (5) Use the 3D maps as frameworks for incorporating structures of various subunits and subcomplexes obtained from X-ray and electron crystallography. (6) Elucidate the spatial relationship between the extrinsic proteins of the Oxygen Evolving Complex and the D1 and D2 proteins of the reaction centre.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search