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Elucidating the structure of photosystem two by electron crystallography

Reference	C11883
Principal Investigator / Supervisor	Professor James Barber
Co-Investigators / Co-Supervisors	Dr Edward Morris
Institution	Imperial College London
Department	Biological Sciences
Funding type	Research
Value (£)	258,700
Status	Completed
Type	Research Grant
Start date	16/10/1999
End date	17/05/2003
Duration	43 months

Abstract

The ultimate objective of this work programme is to solve the structure of photosystem two (PSII) at atomic resolution in order to further our understanding of the mechanisms of its function, especially its ability to use light energy to oxidise water and generate molecular oxygen. Two forms of 2D crystals will be analysed by electron crystallography consisting of the CP47-RC subcomplex and the oxygen evolving core dimer complex. In the case of the former, the present 8 3D map will be improved with the view to obtain 3-4 resolution. The possibility of reconstituting the four atom manganese cluster into the crystals will be explored and if successful, structurally investigated. A 3D structure of the PSII core dimer will also be elucidated at the highest possible resolution with the main aim of identifying the location of the manganese cluster relative to various protein subunits.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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