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Investigation of the factors that control the oligomerisation and shape of a membrane protein

Reference	C11620
Principal Investigator / Supervisor	Krebs Professor of B Christopher Hunter
Co-Investigators / Co-Supervisors	Professor Per Bullough
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	210,276
Status	Completed
Type	Research Grant
Start date	01/12/1999
End date	01/05/2003
Duration	42 months

Abstract

A systematic programme will investigate the molecular interactions leading to the formation of circular membrane protein assemblies. This necessitates an examination of the interactions between membrane-spanning and extrinsic domains of LH2 subunits, which determine the aggregation state and the shape of the complex. This work builds upon our recent success in determining the projection structure of the R. sphaeroides LH2 complex by electron cryomicroscopy. Three aspects of LH2 will be investigated: (1) cofactor binding (2) extrinsic interactions between the N-and C-terminal regions of the LH2 alpha and beta sub-units, and (3) the transmembrane regions of the alpha-subunits. A versatile and comprehensive genetic system, coupled with on-site expertise in spectroscopy and 2D cryo-electron microscopy will be utilised to fulfil the aims of this project.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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