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Functional analysis of the POU domain of the Brn-3b transcription factor
Reference
C11413
Principal Investigator / Supervisor
Professor David Latchman
Co-Investigators /
Co-Supervisors
Institution
University College London
Department
Immunology and Molecular Pathology
Funding type
Research
Value (£)
193,533
Status
Completed
Type
Research Grant
Start date
01/01/2000
End date
01/01/2003
Duration
36 months
Abstract
The three Brn-3 POU family transcription factors play critical but distinct roles in the developing and adult nervous system. Brn-3b is unique however, in having an inhibitory effect on the transcription of specific genes and on neurite outgrowth. This inhibitory effect is dependent on the presence of an isoleucine (I) residue in the POU domain of Brn-3b, substitution with other amino acids converting the mutant Brn-3b into an activator. We will analysis the role of this I residue by determining its effect on the structure of the Brn-3b POU domain by X-ray crystallography and by isolating and characterising other proteins whose ability to interact with Brn-3b is dependent upon the presence of this I residue.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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