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Role of microdomains of ATP and Ca2+ concentration in glucose-triggered insulin secretion
Reference
C11313
Principal Investigator / Supervisor
Professor Guy Rutter
Co-Investigators /
Co-Supervisors
Institution
University of Bristol
Department
Biochemistry
Funding type
Research
Value (£)
158,214
Status
Completed
Type
Research Grant
Start date
01/04/2000
End date
01/04/2003
Duration
36 months
Abstract
Glucose may trigger insulin release from islet beta-cells by increasing the intracellular ATP/ADP ratio. We have established novel bioluminescence imaging technologies with recombinant firefly luciferase to measure free ATP concentrations in single living beta-cells. In this project we will determine the importance of (ATP) changes in the mitochondrial matrix, the mitochondrial inter membrane space and on the surface of secretory vesicles. We will also engineer luciferase in order to measure ATP/ADP ratios in each compartment. Finally we will use targeted fluorescent proteins to assess the importance of the 3- dimensional relationships between the endoplasmic reticulum, the mitochondria, and secretory vesicles.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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