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Regulation of pollen tube growth orientation by calcium dependent protein kinase (CDPK)
Reference
C11308
Principal Investigator / Supervisor
Professor Tony Trewavas
Co-Investigators /
Co-Supervisors
Institution
University of Edinburgh
Department
Inst of Cell and Molecular Biology
Funding type
Research
Value (£)
166,744
Status
Completed
Type
Research Grant
Start date
03/05/1999
End date
03/05/2002
Duration
36 months
Abstract
Pollen tubes must change their orientation of growth in the pistil in order to effect fertilisation and initiate seed formation. Studies by us indicate that cytosolic Ca2+ regulates growth orientation. Furthermore fluorescence ratio imaging of BODIPY-BIM, a protein kinase dye, suggests that relocation of a Ca2+-activated protein kinase activity accompanies the reorientation of the pollen tube growth axis. To test the possibility that this protein kinase is Ca2+ (calmodulin) domain protein kinase (CDPK) we will: (1), over-express CDPK in tobacco pollen and examine the influence of over-expression on BIM distribution, Ca2+ gradients and oscillations. (2), image the distribution of GFP-CDPK and covalently-labelled recombinant CDPK during reorientation. (3), block CDPK activity with specific (caged) autoinhibitory peptides released in different parts of the pollen tube.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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