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Studying cellular dynamics by visualising single molecules within living cells

Reference	C11238
Principal Investigator / Supervisor	Dr Justin Molloy
Co-Investigators / Co-Supervisors	Professor Michelle Peckham
Institution	University of York
Department	Biology
Funding type	Research
Value (£)	291,891
Status	Completed
Type	Research Grant
Start date	01/02/1999
End date	01/04/2002
Duration	38 months

Abstract

Our aim is to increase basic understanding of the movement and interactions of proteins within live, differentiating cells. The method to be employed is evanescent wave, laser excitation of fluorescently-tagged molecules. Tagged-molecules will be present at sufficiently low density that they may be individually imaged with high-sensitivity detectors. The goals of the study are: 1) to monitor the time course of changes in cytoskeletal proteins following cell-cell contact (e.g. founder cell contacts in developing myoblasts); 2) to observe directly the movement of different myosin isoforms along the actin cytoskeleton and to visualise the structures to which they bind; 3) to use correlation analysis to determine the mobility of molecules and sense if they bind to the membrane or cytoskeleton. The proposal is timely as it exploits new imaging technologies and emerging methodologies to answer specific cell biological questions.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	BioImaging (BI) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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