Award details

Identification and cloning of the myeloid cell surface receptor for chaperonin 60

Reference	C11115
Principal Investigator / Supervisor	Professor Brian Henderson
Co-Investigators / Co-Supervisors	Professor Sir Anthony Coates
Institution	University College London
Department	Eastman Dental Institute
Funding type	Research
Value (£)	83,296
Status	Completed
Type	Research Grant
Start date	19/04/1999
End date	19/04/2002
Duration	36 months

Abstract

We have established that chaperonins (cpns) are potent stimulators of myeloid cells able to induce osterclast formation and activation, monocyte cytokine synthesis and dendritic cell activation. We propose the hypothesis that the ability of chaperonins to activate myeloid cells is due to these cells possessing a cell surface receptor(s) for this class of protein. We further hypothesise that the chaperonin receptor or receptors are pattern-recognition receptors and are part of the innate immune response to recognise bacteria. In preliminary studies we have used flow cytometry and plasmon resonance to establish that human monocytes bind cpn 60 with high affinity and using immunoadsorption and western blotting have identified a receptor, or part of a receptor, with a molecular mass of 60kDa. The aims of this application are to characterise this receptor biochemically and, depending on its nature, to clone it using either expression cloning or conventional cDNA cloning. (Joint with grant C11087).

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

C11087 Identification and cloning of the myeloid cell surface receptor for chaperonin 60

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