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Elucidation of the mechanism of myosin ATPase through directed probes
Reference
C11088
Principal Investigator / Supervisor
Professor Clive Bagshaw
Co-Investigators /
Co-Supervisors
Institution
University of Leicester
Department
Biochemistry
Funding type
Research
Value (£)
221,342
Status
Completed
Type
Research Grant
Start date
01/03/1999
End date
10/05/2002
Duration
38 months
Abstract
Engineered Myosin II fragments will be expressed in Dictyostelium cells and their ATPase characterised using spectroscopic and rapid reaction methods. Mutants will be examined in the active site and converter region to follow the path of conformational changes which couple chemical to mechanical events. Single tryptophan mutants and site-directed probes will be introduced at key points in the myosin to detect these changes. The overall goal is to test the predictions concerning the hydrolysis mechanism and swinging lever-arm hypothesis which have arisen from knowledge of the crystal structure of the myosin II motor domain.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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