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Signal transduction pathways and molecular mechanisms involved in the activation of sugar transport by metabolic stress in mammalian cells
Reference
C11058
Principal Investigator / Supervisor
Professor Stephen Baldwin
Co-Investigators /
Co-Supervisors
Institution
University of Leeds
Department
Inst of Molecular & Cellular Biology
Funding type
Research
Value (£)
234,925
Status
Completed
Type
Research Grant
Start date
01/05/1999
End date
01/05/2002
Duration
36 months
Abstract
A characteristic adaptive response on mammalian cells to metabolic stresses such as hypoxia, heat shock, metabolic poisons or hyperosmolarity is a rapid increase in the rate of glucose uptake in order to maintain ATP levels. In many cases, typified by rat Clone 9 cells, this response involves activation of the glucose transporter GLUT1 at the cell surface. The aim of the proposal is to elucidate both the signal transduction pathway(s) involved and the mechanism of transporter activation. A novel procedure for measurement of transport kinetics in single cells will be coupled with microinjection to probe the signalling pathways using inhibitors, dominant negative mutants and antibodies. In parallel, SPR and other approaches will be used to assay and identify proteins that interact with GLUT1 in a stress- regulated fashion and thereby influence its activity.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
BioImaging (BI) [1998]
Funding Scheme
X – not Funded via a specific Funding Scheme
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