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Biosynthesis of mupirocin by Pseudomonas fluorescens

Reference	C11041
Principal Investigator / Supervisor	Professor Christopher Thomas
Co-Investigators / Co-Supervisors	Professor Thomas Simpson
Institution	University of Birmingham
Department	Sch of Biosciences
Funding type	Research
Value (£)	126,584
Status	Completed
Type	Research Grant
Start date	01/06/1999
End date	01/06/2001
Duration	24 months

Abstract

We will complete the nucleotide sequence of the approximately 70 kb region from P. fluorescens NCIB10586 required for biosynthesis of the polyketide antibiotic mupirocin. We will transfer the region into a non-producing strain to determine whether the region is sufficient for complete synthesis. We will express subcloned regions alone to determine whether the prediction that the biosynthesis involves two independent pathways making monic acid and 9-hydroxynonanoic acid. Mutational and chemical/biochemical analysis will be used to correlate genes and biosynthetic pathway. In particular we will clone separately the coding region for the putative thioesterase (TE) which is located in the middle of the cluster. We will inactivate the TE coding region in the chromosome and test for complementation by the cloned TE genetic sequence. We will purify the TE and determine whether it can join the two putative precursors of mupirocin.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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