BBSRC Portfolio Analyser
Award details
The membrane associated domain of the vacuolar H+-ATPase: assembly structure and function
Reference
C10854
Principal Investigator / Supervisor
Professor John B C Findlay
Co-Investigators /
Co-Supervisors
Institution
University of Leeds
Department
Inst of Molecular & Cellular Biology
Funding type
Research
Value (£)
212,636
Status
Completed
Type
Research Grant
Start date
01/04/1999
End date
28/02/2003
Duration
47 months
Abstract
The project will investigate the assembly, structure and function of the vacuolar H+-ATPase, and essential molecular complex of virtually all eukaryotic cells. These acid pumps have bipartite structure, with a soluble catalytic sector connected structurally and mechanistically to a membrane-bound proton translocating sector via stalk subunits. The study will focus on this intramembranous Vo sector and adjacent stalk subunits and will employ techniques in protein expression mutagenesis, chemical cross-linking and labelling, and functional analysis to elucidate the organisation, role and structure of subunits within these sectors. A major aim of the study will be to map the changes in key functional interactions which occur during the catalytic cycle of the pump. The site of binding of natural inhibitors, and the mechanism underlying their action, will also be determined.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search