Award details

Molecular mechanisms of modulation and regulation of the large conductance Ca2+-activated K+ channel

Reference	C10394
Principal Investigator / Supervisor	Professor Alan Williams
Co-Investigators / Co-Supervisors	Professor Andrew Tinker
Institution	Imperial College London
Department	Cardiac Medicine
Funding type	Research
Value (£)	11,652
Status	Completed
Type	Research Grant
Start date	17/05/1999
End date	17/05/2002
Duration	36 months

Abstract

The large conductance Ca2+-activated K+ channel is an important factor in influencing physiological responses as diverse as neuronal firing and smooth muscle tone. Channel activity is influenced by a number of signalling pathways. The mechanisms governing this behaviour have only started to be studied. We aim to examine the molecular basis of regulation of the human cloned channel (alpha and beta subunits) using biochemical and electrophysiological methods in heterologous expression systems namely transiently and stably transfected mammalian cells and Xenopus laevis oocytes. In particular we will study regulation of hSlo currents through the direct action of G-proteins and via protein kinase assays and study the responses of the reconstituted single channel in planar phospholipid bilayers under voltage clamp conditions after the addition of purified kinases. (Joint with grant C10392).

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

C10392 Molecular mechanisms of modulation and regulation of the large conductance Ca2+-activated K+ channel

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